mouse trem2 antibody  (Bio-Techne corporation)

Journal: Alzheimer's & Dementia

Article Title: Sex and life experience shape locus coeruleus pretangle tau pathology

doi: 10.1002/alz.71285

Figure Lengend Snippet: Reduced tyrosine hydroxylase (TH) expression in the hippocampus of htauE14 rats and increased Brain‐Derived Neurotropic Factor (BDNF) expression following early enrichment. (A) Relative optical density (ROD) of TH expression across different groups. N = 4F/4M (cGFP), 6F/4M (cE14), 4F/2M (eSTR.E14), 3F/3M (eER.E14), 5F/3M (lSTR.E14), and 5F/1M (lER.E14). (B) ROD of BDNF expression, showing increased levels with early enrichment. N = 5F/5M (cGFP), 6F/6M (cE14), 6F/2M (eSTR.E14), 4F/4M (eER.E14), 6F/4M (lSTR.E14), and 6F/2M (lER.E14). (C) ROD of glucocorticoid receptor (GR) expression across groups. N = 5F/5M (cGFP), 6F/6M (cE14), 6F/2M (eSTR.E14), 4F/4M (eER.E14), 6F/4M (lSTR.E14), and 6F/2M (lER.E14). (D) ROD of Triggering Receptor Expressed on Myeloid cells 2 (TREM2) expression across groups. N = 5F/5M (cGFP), 6F/6M (cE14), 6F/2M (eSTR.E14), 4F/3M (eER.E14), 6F/4M (lSTR.E14), and 5F/2M (lER.E14). (E) ROD of tumor necrosis factor α (TNFα) expression across groups. N = 4F/5M (cGFP), 6F/5M (cE14), 5F/2M (eSTR.E14), 3F/4M (eER.E14), 6F/3M (lSTR.E14), and 5F/2M (lER.E14). * p < 0.05; ** p < 0.01.

Article Snippet: Membranes were stained with Ponceau S, blocked, and incubated with primary antibodies against brain‐derived neurotrophic factor (BDNF) (1:2000; Abcam, ab108319), glucocorticoid receptor (GR; 1:2000; Thermo Fisher Scientific, MA1‐510), Triggering Receptor Expressed on Myeloid Cells 2 (TREM2; 1:5000; R&D Systems; AF1729), TH (1:5000; Millipore Sigma, MAB318), tumor necrosis factor α (TNFα; 1:2000; Abcam, ab6671), DBH (1:2000; EMD Millipore, MAB508), s262 ptau (1:2000; Abcam, ab92627), T22 (1:2000; Millipore Sigma, ABN454) and Tau5 (1:3000, Millipore, 577801) followed by HRP‐conjugated secondary antibodies.

Techniques: Expressing, Derivative Assay

Journal: bioRxiv

Article Title: Spinal Cord Microglia Exhibit Impaired Repair Responses to Myelin Damage

doi: 10.64898/2026.02.23.707565

Figure Lengend Snippet: (A, C). Representative immunofluorescence images of the medial corpus callosum in the brain and the dorsal region in the lumbar spinal cord stained with antibodies for MerTK and TREM2. (B, D) Mean intensities graphed to depict the comparison between brain and spinal cord. Mean intensities were obtained using Fiji Image J software. Error bars represent standard error of the mean. Statistical analysis was performed using a 3-way ANOVA with Tukey’s post-hoc analysis, n = 5 – 15 where each sample represents a different animal, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p <0.0001.

Article Snippet: The resulting cells were incubated in an FC block solution (Tonbo 70-0161, 1:100) for 10 minutes and then stained in an antibody mix for 30 minutes which consisted of CD45 violetFluor 450 (Tonbo 75-0451, 1:75), CD11b PerCP-Cyanine5.5 (Tonbo 65-0112, 1:75), Brilliant Violet 605 anti-mouse CD16 (Biolegend 158027, 1:75), FITC anti-mouse CD68 (Biolegend 137006, 1:75), APC/Fire 810 anti-mouse CX3CR1 (Biolegend 149054, 1:75), APC anti-mouse F4/80 (Biolegend 123116, 1:75), APC/Fire 750 anti-mouse CD73 (Biolegend 127221, 1:75), PE anti-mouse TREM2 (R&D Systems FAB17291P, 1:75), and viability dye (Tonbo 13-0871, 1:1000).

Techniques: Immunofluorescence, Staining, Comparison, Software